ICP9904 HRP標(biāo)記磷酸化絲氨酸抗體

Product

Description

The affinity purified rabbit polyclonal anti-phospho-serine (anti-pS) was developed using phospho-serine-KLH conjugates and a phospho-protein mixture as the immunogen. The phospho-serine specific antibody was affinity purified with serine-phosphorylated peptide immobilized to agarose. The affinity-purified anti-pS was conjugated to peroxidase (HRP) by reductive amination. Direct application of the primary conjugates will avoid non-specific signals, particularly due to the antibody signal on Western blot analysis of IP's with antibodies from the same species.

Species

Rabbit

Formulation

250 µg/mL in PBS, 50% glycerol

Immunogen

Phospho-serine-KLH and phosvitin mixture

Purification

The antibody was purified with phospho-serine on agarose.

Conjugation

Reductive amination; HRP/Ab molar ratio - 1:10

Specificity

The antibody recognizes proteins phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.

Applications

ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)

Scientific Description

Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to form phospho-serine, which is responsible for various signalling processes in eukaryotes. ^[1]

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

Product Specific References

1. Curr. Opin. Cell. Bio. 2001. 13(2): 131-138. doi:10.1016/S0955-0674(00)00189-7