Z03502 GenCRISPR™ Cas12a (Cpf1) Nuclease
| 參考價(jià) | ￥915.00-￥1600.00 |
- 公司名稱(chēng) 漠寶（廈門(mén)）生物科技有限公司
- 品牌GenScript/金斯瑞
- 型號(hào)Z03502
- 所在地廈門(mén)市
- 廠商性質(zhì)代理商
- 更新時(shí)間2025/8/12 15:18:19
- 訪問(wèn)次數(shù) 96
| 50 μg | 915.00元 | 1 件可售 |
| 100 μg | 1600.00元 | 1 件可售 |
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9min快速濕轉(zhuǎn)儀，10min蛋白染色儀，全自動(dòng)WB系統(tǒng)，預(yù)制膠，WB試劑，Protein A/Ni-NTA/Glutathione/Anti-His/Anti-flag Resin，磁珠，ECL發(fā)光試劑，血清，細(xì)胞，培養(yǎng)基，凋亡檢測(cè)試劑盒，細(xì)胞活力or增殖檢測(cè)試劑盒，細(xì)胞熒光染料，抗體，ELISA試劑盒，重組蛋白，真正無(wú)毒核酸染料，瓊脂糖，qPCR Mix，RT-PCR mix，質(zhì)粒，外泌體解決方案，CRISPR/Cas9試劑等
| 供貨周期 | 一周 | 貨號(hào) | Z03502 |
|---|---|---|---|
| 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,環(huán)保,食品/農(nóng)產(chǎn)品,制藥/生物制藥 |
產(chǎn)品概述
Description：The clustered regularly interspaced short palindromic repeats, known as CRISPR systems are adaptive immune mechanisms commonly present in archaea and bacteria. The CRISPR systems enable the host to specifically target and cleave foreign nucleic acids thus targeting infectiousviruses and plasmids. Recently, a type V CRISPR system has been identified in several bacteria, the Cpf1 CRISPR from Prevotella and Francisella 1. In contrast to Cas9 systems, CRISPR/Cpf1 systems are smaller in size, do not require an additional trans-activating crRNA (tracrRNA), and allow for targeting of additional genomic regions by cleaveing the target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM). On the other hand, the Cas9 system requires a G-rich PAM following the target DNA. Furthermore, Cas12a/Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5’ overhang. Recombinant Acidaminococcus sp. BV3L6 Cas12a (cpf1) nuclease is expressed in E. coli and purified. The nuclease contains nuclear localization sequence (NLS) at the C-terminus and 6× His-tag at the C-terminus.
Key Features：High knockout efficiencies: Consistent high performance in in-vitro plasmid cleavage test.
Time-saving: no need for transcription and translation.
DNA-free: no external DNA added to system.
應(yīng)用指導(dǎo)
crRNA -dependent double-stranded DNA cleavage.
產(chǎn)品屬性
Source：Recombinant Cas12a with an C-terminal NLS expressed by E.coli.
Species：Acidaminococcus sp. (strain BV3L6)
Tag：C-terminal 6× His Tag
Apparent Molecular Weight：~150 kDa, on SDS-PAGE under reducing conditions.
Concentration：4(±10%) mg/ml
Active temperature：This Cas12a is active at 37℃.
Storage Buffer：20 mM Sodium Acetate, 500 mM NaCl, 1 mM TCEP，0.1 mM EDTA, 50% glycerol, PH6.0.
Storage & Stability：This product remains stable up to 18 months at -20℃. Avoid repeated freeze-thaw cycles.
Accession#：U2UMQ6
質(zhì)控參數(shù)
Appearance：Clear, colorless liquid
Purity：≥ 90% by SDS-PAGE
Concentration：4(±10%) mg/m
Bioactivity：≥ 90%
Residual DNase：Undetectable
Residual RNase：Undetectable
Endotoxin Level：≤ 0.1 EU/μg of the protein by gel clotting method
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
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