青霉素G(BP)ELISA快速檢測試劑盒

一、原理

本試劑盒采用間接競爭ELISA方法，在微孔條上預(yù)包被偶聯(lián)抗原，樣本中的殘留物青霉素將和微孔條上預(yù)包被的偶聯(lián)抗原競爭抗青霉素抗體，加入酶標(biāo)記物后，用TMB底物顯色，樣本吸光值與其所含殘留物青霉素的含量成負相關(guān)，與標(biāo)準(zhǔn)曲線比較即可得出相應(yīng)殘留物青霉素的含量。

二、試劑盒特性

u  試劑盒靈敏度：   0.1ppb

u  孵育溫度：            25℃

u  孵育時間：           30min～30min～15min

u  批內(nèi)、批間差： 不超過10%

u  樣本檢測下限

組織  ················································  2ppb

u  交叉反應(yīng)率

青霉素·   ········································  100%

氨芐青霉素········································  0.8%

鄰氯青霉素 ·······································  0.2%

雙氯青霉素 ·······································  0.1%

阿莫西林  ·········································  0.1%

頭孢塞呋  ·········································  0.1%

u  樣本回收率

組  織 ·········································  60%-120%

三、試劑盒組成

四、樣品前處理步驟及檢測分析步驟

  詳見產(chǎn)品說明書。

五、儲藏條件和保質(zhì)期

1、 儲藏條件：試劑盒于2-8℃保存，不要冷凍。

2、 保 質(zhì) 期：該產(chǎn)品有效期為1年，生產(chǎn)日期見包裝盒。

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