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當(dāng)前位置:武漢原生原代生物醫(yī)藥科技有限公司>>技術(shù)文章
當(dāng)前位置:武漢原生原代生物醫(yī)藥科技有限公司>>技術(shù)文章
2021
11-152021
11-11PriCells: Isolation of human primary airway smooth muscle
PriCells:Isolationofhumanprimaryairwaysmoothmuscle1.Primaryculturesofhumanprimaryairwaysmoothmuscle(...2021
11-11PriCells: Isolation of Human Pulmonary Epithelial Cells
PriCells:IsolationofHumanPulmonaryEpithelialCells1.Pulmonaryepithelialcellsfromhumanlungtissuewereis...2021
11-11PriCells: Isolation and growth of pulmonary artery adventitial fibroblasts
PriCells:Isolationandgrowthofpulmonaryarteryadventitialfibroblasts1.Adventitiafromthemainpulmonaryar...2021
11-11PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells
PriCells:PrimaryCultureofPulmonaryArterialSmoothMuscleCells1.PrimaryculturesofPulmonaryArterialSmoot...2021
11-11PriCells: Measurement of primary endothelial cell permeability to fluxes of dextran or albumin
PriCells:MeasurementofprimaryendothelialcellpermeabilitytofluxesofdextranoralbuminThefluxesofalbumin...2021
11-10大鼠肝癌細(xì)胞常見(jiàn)問(wèn)題及注意事項(xiàng)
大鼠肝癌細(xì)胞常見(jiàn)問(wèn)題及解決:細(xì)胞漂?。号囵B(yǎng)瓶不開(kāi)封,瓶口酒精擦拭后平躺放置在培養(yǎng)箱。次日觀察,如細(xì)胞大部分又貼回瓶底,表明細(xì)胞活力正常,剩余漂浮的細(xì)胞可以離心去掉,留10ml培養(yǎng)液培養(yǎng)觀察,細(xì)胞生長(zhǎng)至...2021
11-10PriCells: Isolation and Culture of Human Brain Tumor Stem Cell
PriCells:IsolationandCultureofHumanBrainTumorStemCellTheisolation,culture,identification,andpurifica...2021
11-10PriCells: Identification and expansion of the tumorigenic lung cancer stem cell population
PriCells:IdentificationandexpansionofthetumorigeniclungcancerstemcellpopulationLungcancercontainsara...2021
11-10PriCells: Isolation and Culture of Human Liver Stem Cells
PriCells:IsolationandCultureofHumanLiverStemCellsMaterialsandMethods1.Humanhepatocyteswereisolatedfr...2021
11-10PriCells: Isolation and Culture of Multipotent Stem Cells from Human Bone Morrow.
PriCells:IsolationandCultureofMultipotentStemCellsfromHumanBoneMorrow.Bonemarrowcontainsthreetypesof...2021
11-09PriCells: Ex Vivo Human Primary Mesenchymal Stem Cells (MSCs) Culture
PriCells:ExVivoHumanPrimaryMesenchymalStemCells(MSCs)CultureHumanbonemarrowcontainsmesenchymalprogen...2021
11-08PriCells: 正常大鼠施旺細(xì)胞的培養(yǎng)
PriCells:正常大鼠施旺細(xì)胞的培養(yǎng)實(shí)驗(yàn)材料:1.生后2d大鼠的坐骨神經(jīng);2.不含Ca2+和Mg2+的1×PBS,添加200000IU/L青霉素、200mg/L鏈霉素,pH7.2;3.消化液:0....2021
11-08PriCells: 正常大鼠小膠質(zhì)細(xì)胞的培養(yǎng)
PriCells:正常大鼠小膠質(zhì)細(xì)胞的培養(yǎng)實(shí)驗(yàn)材料:1.新生大鼠或小鼠;2.不含Ca2+和Mg2+的1×PBS,添加200000IU/L青霉素、200mg/L鏈霉素,pH7.2;3.培養(yǎng)用液:含10%...2021
11-08PriCells: 標(biāo)記的鏈霉卵白素-生物素法(LSAB)
PriCells:標(biāo)記的鏈霉卵白素-生物素法(LSAB)標(biāo)記的鏈霉卵白素-生物素法(LSAB法)是標(biāo)記的卵白素技術(shù),20世紀(jì)80年代開(kāi)始采用?;驹噭孩俚谝豢贵w;②生物素化第二抗體;③辣根過(guò)氧化物酶...2021
11-08PriCells: 免疫酶細(xì)胞化學(xué)技術(shù)中的雙PAP法檢測(cè)微量抗原
PriCells:免疫酶細(xì)胞化學(xué)技術(shù)中的雙PAP法檢測(cè)微量抗原在復(fù)合物體系中連接更多的PAP復(fù)合物,來(lái)進(jìn)一步放大抗原,從而使靈敏度更為增強(qiáng),適用于檢測(cè)微量抗原。實(shí)驗(yàn)方法:1.將固定后的標(biāo)本用PBS漂洗...2021
11-08PriCells: 原代細(xì)胞培養(yǎng)后過(guò)氧化物酶檢測(cè)
PriCells:原代細(xì)胞培養(yǎng)后過(guò)氧化物酶檢測(cè)試劑和器材:1.過(guò)氧化物儲(chǔ)存液:30mmol/L過(guò)氧化氫儲(chǔ)存在T-牛血清白蛋白(Tris-牛血清白蛋白溶液)中;2.樣品緩沖液:2體積的T-牛血清白蛋白溶...2021
11-05PriCells: 原代細(xì)胞培養(yǎng)后,碘克沙醇梯度溶液分析主要細(xì)胞器
PriCells:原代細(xì)胞培養(yǎng)后,碘克沙醇梯度溶液分析主要細(xì)胞器試劑和器材:1.OptiPrepTM(600g/L的碘克沙醇);2.勻漿介質(zhì):0.25mmol/L蔗糖溶液、1mmol/L乙二胺四乙酸、...2021
11-05不連續(xù)Nycodenz梯度溶液中原代細(xì)胞溶酶體的分離
PriCells:不連續(xù)Nycodenz梯度溶液中原代細(xì)胞溶酶體的分離試劑和器材:1.勻漿介質(zhì):0.25mol/L蔗糖溶液、1mmol/L乙二胺四乙酸、10mmol/LHepes-NaOH,pH7.4...2021
11-05哺乳動(dòng)物原代細(xì)胞培養(yǎng)的β-半乳糖苷酶(分光光度測(cè)定)
PriCells:哺乳動(dòng)物原代細(xì)胞培養(yǎng)的β-半乳糖苷酶(分光光度測(cè)定)試劑和器材:1.反應(yīng)緩沖液:5g/L聚乙二醇辛基苯基醚(TritonX-100)、0.05mol/L檸檬酸鈉-磷酸鹽緩沖液,pH4...請(qǐng)輸入賬號(hào)
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